LOCUS       Exported                3606 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0062_sensor_pluxRcr12yRBS-sfGFP
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    127..196
                     /note="spacer (B0040)"
     promoter        197..251
                     /note="lux rR (R0062)"
     3'UTR           252..303
                     /note="crR12y"
     RBS             286..297
                     /note="RBS (B0034)"
     CDS             304..1029
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     terminator      1032..1148
                     /note="T1"
     terminator      1041..1127
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1155..1174
                     /note="AB179"
     primer_bind     1340..1359
                     /note="AB5"
     rep_origin      1493..2320
                     /direction=RIGHT
                     /note="p15A"
     rep_origin      1741..1743
                     /direction=RIGHT
                     /note="P15 ori from pACYC184"
     rep_origin      complement(1744..2288)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     2392..2411
                     /note="AB180"
     oriT            2422..2530
                     /note="incP origin of transfer"
     CDS             complement(2686..3501)
                     /note="KamR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagaaggt tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cctcttaaga
      181 ggtcactgac ctaacaacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat
      241 agtcgaataa agaattctac cattcacctc ttggatttag ctattaaaga ggagaaaggt
      301 accatgagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      361 ggtgatgtta atgggcacaa attttctgtc cgtggagagg gtgaaggtga tgctacaaac
      421 ggaaaactca cccttaaatt tatttgcact actggaaaac tacctgttcc gtggccaaca
      481 cttgtcacta ctctgaccta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa
      541 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct
      601 ttcaaagatg acgggaccta caagacgcgt gctgaagtca agtttgaagg tgataccctt
      661 gttaatcgta tcgagttaaa gggtattgat tttaaagaag atggaaacat tcttggacac
      721 aaactcgagt acaactttaa ctcacacaat gtatacatca cggcagacaa acaaaagaat
      781 ggaatcaaag ctaacttcaa aattcgccac aacgttgaag atggttccgt tcaactagca
      841 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      901 tacctgtcga cacaatctgt cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc
      961 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaaagg
     1021 ccttaataaa ttaaaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1081 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga
     1141 cagctgggcg cgccaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1201 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
     1261 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
     1321 tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt
     1381 ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg
     1441 ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct
     1501 tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg
     1561 ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg
     1621 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag
     1681 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc
     1741 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     1801 gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccctggcgg ctccctcgtg
     1861 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     1921 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     1981 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2041 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
     2101 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
     2161 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
     2221 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
     2281 gatctcaaga agatcatctt attaatcaga taaaatattt ctagcatgag cggatacata
     2341 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2401 ccacctgcta gggccggcct acggccagcc tcgcagagca ggattcccgt tgagcaccgc
     2461 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
     2521 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
     2581 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
     2641 gaagcagggt tatgcagcgg aaaaggaccc ttgtccaatt aattattaga aaaattcatc
     2701 cagcatcaga tgaaattgca gtttgttcat atccggatta tcaatgccat atttctgaaa
     2761 cagacgtttt tgcaggctcg ggctaaattc gcccaggcag ttccacagaa tggccagatc
     2821 ctgataacga tccgcaatgc ccacacggcc cacatcaatg cagccaatca gtttgccttc
     2881 atcgaaaatc aggttatcca ggctaaaatc gccgtgggtc accacgctat ccgggctaaa
     2941 cggcagcagt ttatgcattt ctttccacac ctgttccacc ggccagccgt tacgttcatc
     3001 atcaaaatcg ctcgcatcca ccaggccgtt gttcatacgg ctctgcgcct gggccagacg
     3061 aaacacacga tcgctgttaa acgggcagtt gcacaccgga atgctatgca gacgacgcag
     3121 aaacacggcc agcgcatcca caatgttttc gccgctatcc ggatattctt ccagcacctg
     3181 aaacgcggtt ttgcccggaa tcgcggtggt cagcagccac gcatcatccg gggtgcgaat
     3241 aaaatgttta atggtcggca gcggcataaa ttcggtcagc cagttcagac gcaccatttc
     3301 atcggtcaca tcgttcgcca cgctgccttt gccatgtttc agaaacagtt ccggcgcatc
     3361 cggtttgcca tacagacgat aaatggtcgc gccgctctga cccacgttat cacgcgccca
     3421 tttatagcca tacagatccg catccatgtt gctgttcaga cgcggacggc tacagctcgt
     3481 ttcacgctga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     3541 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     3601 aaattt
//
